Composite

Part:BBa_K3805924

Designed by: Han Yu   Group: iGEM21_BNU-China   (2021-09-30)


T7-RBS1- AgrC-T7 terminator-RBS2- AgrA-T7Te terminator

We combined AgrC(BBa_K3805508)[1] and AgrA(BBa_K3805525[2] together with T7 promoter and RBS, in order to form the two component system that can manipulate the activation of P2.promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None